Tip 1: Add phosphatase inhibitors to lysis buffers for extraction of phosphorylated proteins. Homogenize thoroughly and keep the sample on ice for 30 min. In general, add 500μl RIPA buffer for approximately every 10 mg of tissue. Transfer the tissue to a homogenizer and add RIPA buffer with protease inhibitor. Tissues:ĭissect the tissue of interest and wash briefly with chilled 1X PBS to remove any blood if necessary, cut the tissue into smaller pieces whilst keeping it on ice. Vortex to mix and keep on ice for 30 min, vortexing occasionally. Datasheets that this protocol applies to All those where western blots using goat polyclonal antibodies on tissue lysates (human, rat or mouse) are described. NP-40 buffer 150 mM NaCl 1. Reduce the volume of RIPA buffer accordingly if a higher protein concentration is required. Lysis buffers These buffers may be stored at 4☌ for several weeks or aliquoted and stored at -20☌ for up to a year. Inefficient lysis can result in incomplete isolation and. In general, add 100μl RIPA buffer for approximately every 106 cells present in the pellet (count cells before centrifugation). Efficient cell lysis is an important step in preparing quality samples for Western blot analysis. Wash 3 times with ice-cold 1X PBS and then add chilled RIPA buffer with protease inhibitor. Pellet the cultured cells by centrifugation for 5 minutes at 1000 x g (approximately 2000 rpm) at 4☌. Cells from 6 well plates should be lysed in 750 L to 1 mL of WLB with protease tablets. Pre-cool a refrigerated centrifuge to 4☌. Sample Preparation: Cell Lysate and Conditioned Media Cells from 10 cm plates are are collected at 100 confluence, Day 0, in 1 mL of Western Lysis Buffer (WLB) and stored at -20 C. Dependent on the location of the protein of interest, a different lysate buffer is needed to obtain a high yield and purity of the protein. Lysate buffers contain different detergents that help to release soluble proteins (Triton-X, Tween, SDS, CHAPS). However, if the objective is only western blot, there is no need to sonicate cells because boiling in Laemmelli buffer for 3 minutes should generally denature and release any type of protein. Heat the mixture to 95☌ for 5 minutes before loading onto an SDS-PAGE gel.Ĭell lysis is the breaking down of the cell membrane and the separation of proteins from the non-soluble parts of the cell. For Western blotting, mix sample with 4X SDS sample buffer to a final dilution of 1X.Samples can be frozen at -80☌ for long-term storage, or be used for immediate Western blotting or immunoprecipitation.Determine protein concentration of the lysate by Bradford or BCA protein assay.Centrifuge at 10,000 x g (approximately 9700 rpm for rotors of a 9.5 cm radius) for 20 minutes at 4☌ to pellet cell debris, and then transfer the supernatant to a fresh microfuge tube without disturbing the pellet.Tip 2: The addition of DNase for DNA digestion is not recommended as this introduces protein contamination from the enzyme. Keep the sample on ice during the sonication. Adjust sonication time to your type of sample: 1 min for cell lysates and 2–5 min for tissue lysates at a power of about 180 watts (in rounds of 10 seconds sonication/10 seconds rest for each cycle). Sonicate the sample to break the cells or tissue up further and to shear DNA.
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![]() The developers of adobe premiere take care of the updates to make it more responsive and user-friendly with time. You will get direct publishing option once you are done editing the video.Īdobe premiere’s interface allows users to add their productivity, efficiency, and effectiveness in the video. You can also try its video titling and graphics.Į) It supports multiple format files like OGM, WMV, MPEG, FLV, MKV etc.į) It has labels, Libraries and type tool to help you out while editing. You can add multiple effects to the video and make it worth watching.ĭ) There are many transition templates available. Key features of adobe premiere pro are:Ī) You can handle simultaneous projects in it.ī) It consists of many motion graphics templates to create a creative video.Ĭ) It has an option for VR editing, VR rotate sphere and VR audio editing. You need to get familiar with the software first to know about all the tools due to the wide range of functions in it.Īdobe premiere has very smooth editing tools. It is a professional video editing software. You must have noticed many Youtubers and bloggers use this software for editing their vlogs and videos. It can increase the quality of video in so many ways. Your video will be saved after this step.Īdobe Premiere Pro is widely used and powerful video enhancing software. After editing click on convert to export the video. Edit and exportĪfter editing and enhancing the video quality, you can use the other tools to edit your video file like adding watermarks, rotating, crop or adding effects, etc. Third is remove video noise which gives a clear video after editing.Īnd the last is reduces video shaking which assists you to adjust the whole frame moves and get a stable video. Second is Optimize brightness and contrast which is a dedicated section for making your video color more enjoyable. You will find four options to improve the video, first upscale resolution where you can increase the quality of video to HD. Search for “Video enhance” option in the main interface for enhancing video. Open the software on your laptop, click add file option to import the video files for editing or enhancing. Add video to the aiseesoft video enhancer. Steps to enhance the video on computer S1. This feature is applied automatically if the software finds the video dull.Ĥ) You can also crop, rotate, flip and add watermark to the video easily with the help of tools.ĥ) The most important feature, it can reduce the video shaking to get a stable video. Key features of Aiseesoft:ġ) You can easily upscale the video resolution to 1080 HD or 4K.Ģ) You can remove unwanted noise to get a clear vision of the video.ģ) Aiseesoft can also optimize the brightness and contrast of the video. It is available for free for Windows and Mac operating system. It is the combination of video enhancer and video converter tool. Aiseesoft Video EnhancerĪiseesoft is professional video enhancer software available for both Windows and Mac. This article will give you a list of best video enhancer software lists which you can use to increase the quality of your videos.Ĥ 4. You can use this software on android phones, windows, Mac, etc. There are many video enhancer software developed just to resolve this issue. Or, you can try editing videos on your own. The first variant you can choose is to send your files to video editing services, describe your demands, and wait till experts will make all the needed corrections. The question arises can we improve the quality of the video? It cannot be worse than you find any old video blurry and shaky. Videos bring joy and happiness to your life.īut what if the video you have shoot is in poor quality? Videos can speak more than words or pictures. This article lists the best video enhancing software for you. You can also add watermarks, subtitles or covert the you can either double the speed of the video or slow down the video. You need to get familiar with the software first to know you can upscale 480p to 720p or 1080p, convert from sd to an hd video. Remove unwanted noise to get adobe premiere pro is widely used and powerful video enhancing software. You can upscale the video resolution to 1080 hd or 4k. Video enhancer software is available on android phones, windows, mac, etc. ![]() Running the Jupyter notebook command will open the notebook page in the new tab of the browser as localhost. Jupyter creates the localhost server to execute the Python programs. Running Jupyter Notebook python -m notebook Running Jupyter Notebook python3 -m notebook Python 2 python -m pip install -upgrade pipĬommands for installing Python packages for Python 2 versions are pretty straightforward. The older Python 3 version has the tool pip3. ![]() Remember, pip is a package manager tool by default comes with Python binaries. py -m notebook Python 3 (older versions) python3 -m pip3 install -upgrade pip Once you installed it, run the following command to run the notebook. Learn more about managing Python packages using Pip. Note: Managing the Python module is crucial for Python developers. It is always good practice to upgrade pip before installing any Python module. Here, the first command is to upgrade the pip. Python 3.7 py -m pip install -upgrade pip You can install the Jupyter Notebook from the Python standard library with simple commands. It provides an OS-independent system, so you can use it for any of the operating systems like Windows, Linux (Ubuntu), macOS, etc… Pip is the default package management system or tool for installing/uninstalling and managing different packages in Python. Installing Jupyter Python Notebook For Python 2 and 3 ![]()
![]() ![]() Analyze your skin tone thanks to a unique 360 selfie video technology powered by AI, to find your shade match and preferred foundation coverage and finish. Discover the ideal shade of foundation for your skin tone with NARS Foundation Shade Finder. Prestige Beauty Total Department/Specialty, Makeup Sales, January 2018–December 2021. Your unique skin type has finally met its perfect match NARS. 90% agree this product leaves a creaseless finish**.92% agree this product reduces visible signs of fatigue** Explore official NARS foundation virtual try-on tool to find your foundation formula and shade match.93% agree this product brightens up the eye area**.95% agree this product smooths out the surface of skin**. ![]() consumer perception study of 60 women after two weeks of use.Hot-Selling Products HP All In One printer OfficeJet 6210 / HP Officejet 6812e-All-in-One Printer / HP OfficeJet 6835 e-All-in-One Printer / HP OfficeJet 6954 All-in-One Inkjet Printer / HP Officejet 7000 Wide Format Printer / HP OfficeJet 7510 Wide Format All-in-One Printer / HP OfficeJet 8015 All-in-One Printer / HP OfficeJet H470 Mobile Printer - Black / HP OfficeJet Pro 6830 Wireless All-in-One Photo Printer / HP OfficeJet Pro 6830 Wireless All-in-One Photo Printer / Epson EcoTank Pro ET-16600 Inkjet Printer / Epson Eco Tank Pro ET-5880 Printer / Epson Stylus NX230 Inkjet printer / INTERGREAT Portable Washing Machine, 17.6 lbs Mini Small Laundry Washer Combo with Spin Dryer / Zerodis Portable Washing Machine, 120W Mini Washer / Flexzion Electric Air Fryer Cooker 1400W (Black) / Oster DiamondForce Nonstick XL 5 Quart Digital Air Fryer & Touchscreen Blender / PowerXL Vortex Air Fryer Plus 5 Quart / SMALL FISH Air fryer Yellow Oilless Oven / QINQIWD 2.5L Pink Air Fryer / GREECHO Air Fryer and Frying Pan Combo / Sun Terriory 4.8Quart (4.5 Liter) Air Fryer / Bella Pro Series 4-qt. 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It seems that NARS is paying tribute to late musical icon Prince with some of the names of their new Velvet Matte shades. Neither have been a perfect match, and both have pulled slightly yellow, but the Tarte is a better match than the Nars both in color and shade. On Valentine’s Day, NARS added ten new shades of their best-selling, cult favorite Velvet Matte pencils. We noticed something interesting about the names. Because of this, Ive been using the Tarte exclusively for. DynaGlyde® additive technology - Royal Purple’s high film strength, noncorrosive DynaGlyde additive technology contains special anti-wear additives, oiliness properties and cushioning molecules to provide the lubricity necessary to excel in worm gear lubrication.Ĭ. Royal Purple’s noncorrosive, extreme pressure (EP) gear oils and greases contain Synslide additive technology.ī. Synslide® additive technology - This exclusive additive technology offers all of the performance advantages of Synerlec additive technology plus superior protection against boundary lubrication (lubrication between two rubbing surfaces without development of a full-fluid lubricating film) conditions typically caused by heavy loads, shock loads and slow operating speeds. In addition to Synerlec additive technology, Royal Purple formulates lubricants using the following three additional additive technologies:Ī. Royal Purple’s synthetic lubricants rapidly and completely separate from water, allowing water to easily be drained from the bottom of the oil reservoir. ![]() Many oils form milky oil-water emulsions, which greatly shortens the life of both the oil and the equipment it lubricates. Synerlec’s tough film strength protects during operation, acts as a preservative oil during shutdown and provides instant lubrication upon startup until a full fluid oil film is established. Royal Purple’s Synerlec additive technology ionically bonds with all metal surfaces displacing moisture. Synerlec additive technology provides superior protection against rust and corrosion in both salt and freshwater environments. Equipment will remain cleaner and will have longer life. This greatly increases oil life, reducing the amount of oil purchased and disposed of. Synerlec additive technology protects against oil oxidation, which causes oils to break down, to thicken and to form corrosive acids, sludge, varnish or lacquer deposits in equipment. coggygria 'Kromhout'.Royal Purple’s oils formulated with Synerlec additive technology last considerably longer than other mineral and synthetic oils. 'Royal Purple' was raised at Lombarts Nursery in Boskoop, Holland. Cotinus is in the same family as and closely related to the sumacs ( Rhus). Foliage retains good color without much fading throughout the growing season. Ovate to obovate leaves (to 3” long) emerge a rich maroon red in spring, and then gradually mature to dark purplish-red to purplish-black in summer. But it is the foliage that particularly distinguishes 'Royal Purple'. As is the case with all plants of this species, it gets its common name of smoketree (or smokebush) not from the tiny, insignificant, yellowish flowers which appear in branching, terminal panicles (6-8” long) in spring, but from the billowy hairs (attached to elongated stalks on the spent flower clusters) which turn a smoky pink to purplish pink in late spring, thus covering the plant with fluffy, hazy, smoke-like puffs throughout summer. It is a deciduous, upright, loose-spreading, multi-stemmed shrub that typically matures over time to 10-15' tall. ‘Royal Purple’ is a compact, purple-leaved version of the European species. Specific epithet comes from the Greek word kokkugia meaning smoke tree. ![]() Genus name comes from the Greek word kotinus meaning olive. ![]() Fall color is highly variable, but at its best produces attractive shades of yellow, orange, and purplish-red. Bluish green leaves (to 3” long) are ovate to obovate. It gets its common name of smoketree (or smokebush) not from the tiny, insignificant, yellowish flowers which appear in branching, terminal panicles (to 6-8” long) in spring, but from the billowy hairs (attached to elongated stalks on the spent flower clusters) which turn a smokey pink to purplish pink in summer, thus covering the tree with fluffy, hazy, smoke-like puffs throughout summer. ![]() It typically matures over time to 10-15’ tall and as wide. Cotinus coggygria, commonly known as smoketree, is an upright, loose-spreading, multi-stemmed, deciduous shrub that is native from southern Europe to central China.
![]() John Lennon claimed to Playboy that he kicked in the middle eight himself, although Paul disagrees. “And I Love Her” was authored primarily by Sir Paul McCartney. “West Coast” steals … er … samples, no … interpolates (that’s it!) the opening riff from the Beatles’ 1964 song “And I Love Her” for the beginning of its slower-tempo second section. It’s meant for swaying on balconies, lounging in beds, and driving real slow. Here are some of the songs I thought about during my first few listens to “West Coast,” all of which got me even more excited about the prospect of Ultraviolence. ![]() Like Lana’s first breakout song, “Video Games,” it isn’t intended for raging at the clob. (Side note: Is she singing about Megan Draper?) “West Coast” shares musical DNA with a lot of songs that qualify for the highest West Coast title of honor: low-rider oldie. Singing about leaving a lover for the Californian promise of fame, Lana sounds more sinister and sensual than ever. It’s a logical evolution of her prior sound. The song deviates from the winning formula Del Rey invented and then burnished with Born to Die and its Paradise edition, without fully shedding her old skin.Īs with all Lana songs, the lyrics to “West Coast” are both incredibly specific and obstructively vague, and here their dreamlike quality complements the druggy instrumentation. The result is “West Coast,” a four-and-a-quarter-minute spell produced by Dan Auerbach from the Black Keys. ![]() A song that leaked recently, called “Meet Me in the Pale Moonlight,” turned out to be four years old, and Lana promised her new music would sound nothing like any old demos. This weekend, Lana Del Rey finally dropped the first single from her upcoming album Ultraviolence, after teasing fans with Sunset Strip billboards and incomplete first couplets. ![]() Archived (PDF) from the original on Septem. ^ a b "2012 NFL Record and Fact Book" (PDF).^ "Miami's Bush Eyeing NFL Rushing Title".List of NCAA major college football yearly rushing leaders.List of National Football League career rushing yards leaders.NOTE: Italics denotes season in which a player won the AAFC rushing title. List of AFL rushing title winners įurther information: All-America Football Conference All-American Football Conference (AAFC) rushing title winners by season Why Cam Newtons NFL Record for QB Rushing Touchdowns Is a Fluke Michael Vick (33) is the all-time leader in rushing yards by a quarterback (5,219 yards). National Football League (NFL) rushing title winners by season Season + Indicates Hall of Famer, season age in parentheses. Single game leaders only reflect years for which we have complete boxscore data (1950 onward), all playoff games, and all 100 yard rushing/receiving or 300 yard passing games from 1940-1959. Player won the AP Offensive Player of the Year award in the same year All-Time Rushing & Receiving Touchdowns Leaders. ![]() Career Passing Yards, Single Season Rush TD, Single Game Sacks. Player won the AP Most Valuable Player award in the same year Single game leaders only reflect years for which we have complete boxscore data (1950 onward), all playoff games, and all 100 yard rushing/receiving or 300 yard passing games from 1940-1959. Derrick Henry won back-to-back rushing titles in 20, and is the most recent player to do so. Adrian Peterson led the league in rushing in the 2008, 2012, and 2015 seasons. LaDainian Tomlinson won back-to-back rushing titles in 20. View 2021 NFL Rushing stats, Rushing leaders & player Rushing statistics. Other Finders: Versus Finder, Touchdown Finder, Field Goal Finder, Game Play Finder, Drive Finder. ![]() > NFL Rushing Yards Year-by-Year Leaders. Emmitt Smith was the league's rushing champion four times, including three consecutive years from 1991 to 1993. For some seasons (1960-1969 & 1947-1950). Eric Dickerson led the league in rushing in 19, his first two seasons in the league, and won two more titles in 19. Gale Sayers led the NFL in rushing in 19. List of NFL rushing title winners Byron "Whizzer" White, a future associate justice of the US Supreme Court, won the league rushing title in 19. In 2022 the NFL announced that, "To permanently honor the impact of Jim Brown on the NFL, the player with the most rushing yards each season will be presented with the " Jim Brown Award". Derrick Henry is the most recent player to win back-to-back titles. The most recent rushing champion is Josh Jacobs of the Las Vegas Raiders, who led the league with 1,656 yards rushing over the 2022 season. The Cleveland Browns have recorded the most rushing titles with eleven the Dallas Cowboys rank second, with seven rushing titles. Steve Van Buren, Emmitt Smith, and Earl Campbell each recorded three consecutive rushing titles. Jim Brown also holds the record for the most consecutive rushing titles with five, having led the league in rushing each year from 1957 to 1961. Simpson, Steve Van Buren, and Barry Sanders are tied for the second-most rushing titles, each having won four times. The player with the most rushing titles is Jim Brown, who was the rushing champion eight times over his career. Simpson in 1973 and most recently achieved by Derrick Henry in 2020. Eight rushing champions have recorded over 2,000 rushing yards, a feat first accomplished by O. Since the adoption of the 14-game season in 1961, all but one rushing champion have recorded over 1,000 yards rushing. ![]() The NFL did not begin keeping official records until the 1932 season. NFL team ranking by all-time regular season winning percentage 2023. In addition to the NFL rushing champion, league record books recognize the rushing champions of the American Football League (AFL), which operated from 1960 to 1969 before being absorbed into the National Football League in 1970. Kansas City Chiefs all-time leaders number of touchdowns scored from 1960 to 2023. In the National Football League (NFL), the player who has recorded the most rushing yards for a season is considered the winner of the rushing title for that season. A rush, also known as a running play, generally occurs when the quarterback hands or tosses the ball backwards to the running back, but other players, such as the quarterback, can run with the ball. NFL footage © NFL Productions LLC.In American football, rushing and passing are the two main methods of advancing the ball down the field. All other NFL-related trademarks are trademarks of the National Football League. NFL and the NFL shield design are registered trademarks of the National Football League.The team names, logos and uniform designs are registered trademarks of the teams indicated. It also works with unofficial controllers. ![]() In the case of DualShock 4, Bluetooth version 2.1 or higher is required. In order to use DualShock 3, Bluetooth 2.0 or higher is required, and it must support EDR (Enhanced Data Rate). NET Framework 4.5, Microsoft Visual C++ 2010 Redistributable Package, Microsoft Visual C++ 2013 Runtime, and DirectX Runtime. If you have Windows 8 or later, this step isn't necessary, as they come with the computer by default. I had this problem with Tracer brand PS3 controller. Therefore, you need to have the Xbox drivers installed. Scp Virtual Bus Driver Bluetooth Host (ScpToolkit) DualShock 3 Controller (ScpToolkit) DualShock 4 Controller (ScpToolkit). 836 Share 121K views 2 years ago In this video, I show how to fix bluetooth connection problem although you install everything properly. This program acts as an intermediary between the controller and the games, making the games believe that you're using an Xbox controller. To make it work, you need a wide variety of tools. Bluetooth disappeared from PC after installing ScP Toolkit to connect PS3 controller By mato200 May 11 in Troubleshooting Followers 1 mato200 Member 23 Posted May 11 I am using win11 desktop with this BT dongle: Hoco USB Bluetooth 5.0 Dongle Also tried installing official drivers. This tool may cause driver problems that force you to reinstall some of them from scratch, such as Bluetooth drivers.įrom SCPToolkit, you can add wired or Bluetooth controllers. ![]() SCPToolkit is somewhat more complex to use than DS4Windows. However, some people still prefer to use SCPToolkit on earlier versions of Windows. The most advanced tool available is DS4Windows, which is compatible with DualShock 3, DualShock 4, and DualSense. SCPToolkit is a driver that allows you to use PlayStation 3 and PlayStation 4 controllers on Windows. When you run the scp toolkit driver installer (see that neat picture below), you have to make sure to plug in the ps3 controller first, have then 'install dualshock 3 driver' checkbox selected and right next to it on the arrow symbol 'choose dualshock 3 controllers to install', you may have to scroll down in the list there until you find your c. ![]() The image below illustrates startup benefits you can expect from Ngen.exe: 2. Nonethless, you still need to run Ngen.exe manually, because Native Image Task only works with assemblies located in GAC or Windows Store app package. NET Framework 4.5+ applications when a computer is idle. ![]() Windows 8 (and newer versions of the Windows OS) include a Native Image Task that automatically generates native images for frequently used. For example, a user cancelled the UAC prompt. Run the process and save the executable’s hash: Var ngenPath = Path.Combine(dotNetRuntimePath, "ngen.exe") Īrguments = $"install \"\" /nologo", Var dotNetRuntimePath = RuntimeEnvironment.GetRuntimeDirectory() Var hash = string.Concat(SHA1.Create().ComputeHash(File.ReadAllBytes(assemblyLocation)) Cancel the operation if the application does not have changes: Var hashPath = Path.Combine(Environment.GetFolderPath(), "hash.txt") Create this file or load the saved hash if the file already exists: Specify a path to the file that stores your executable’s hash. Var assemblyLocation = Assembly.GetEntryAssembly().Location To only run Ngen.exe for the first startup, calculate a hash for your executable file and check this hash during subsequent startups: If you distribute your application as Click Once or an archive (and don’t have an installer), you can call Ngen.exe from code during application startup. You’ll need to execute the following line during the installation process and Ngen.exe will automatically process all assemblies associated with your project: C:\Windows\Microsoft.NET\Framework\v9\ngen.exe install C:\MyApp.exe The best way to run Ngen on a user’s machine is to incorporate Ngen.exe within your application installer. You can run it on your machine to test performance, but to optimize cold start for an end user, you need to use Ngen.exe on the user machine. It’s important to note that Ngen.exe should be used on the machine where your application will be used. You can use Ngen.exe to generate native image libraries that already contain native code. Translation from MSIL to machine code begins at startup - a process that may require significant time. This code needs to be translated to machine code before executing the application. NET Framework projects produce assemblies with Microsoft Intermediate Language (MSIL) code. NET Framework app (.NET/.NET Core projects don’t support Ngen). Native Image Generator (Ngen.exe) is the first tool you should consider when optimizing startup for a. Use LoadingDecorator for “Heavy” UI ElementsĪ final technique involves the use of Visual Studio's Performance Profiler (helps determine the underlying cause of performance-related issues).The following techniques are specific to DevExpress WPF UI Controls: The 3 techniques listed below apply to all WPF apps, regardless of components used: This post will document 9 strategies to improve cold start performance for your WPF app. |
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